Converting a conventional fluorescence microscope into a high-performance platform for sub-10-nm single-molecule localization microscopy

We present an open-source approach to convert a conventional fluorescence microscope into a high-performance single-molecule localization microscopy (SMLM) platform, achieving sub-10-nm localization precision. This website provides a step-by-step guide to replicate the system, validate each module, and adapt it to your own laboratory setup.