Hardware

From a commercial fluorescence microscope to a fully functional single-molecule localization microscopy (SMLM) platform, including key optical modules.

Commercial vs converted microscope
Commercial microscope vs converted SMLM system
TIRF / HiLO illumination module
TIRF / HiLO module
Focus lock module
Focus lock module
3D imaging module (astigmatism-based)
3D astigmatism module

Results

Representative datasets acquired with the system, including calibration standards and biological samples

DNA origami calibration structures (2D grids and 3D tetrahedrons)
DNA origami grids and tetrahedrons
Four-target imaging on neuronal cultures.
4-color neuronal culture imaging